ARBOR ASSAYS OFFERS DISCOUNTED REAGENTS & ISWE MINI KITS
ISWE and Arbor Assays have partnered to develop and sell kits for measuring common endocrine hormones to the wildlife endocrinology community. Currently, kits are available for testosterone, progesterone, 17 beta-estradiol, cortisol, and corticosterone. The Progesterone Mini-kit uses the CL425 monoclonal antibody, while the other mini-kits use antibodies developed and owned by ISWE. The kit reagents are supplied with both the antibody and conjugate as 50x concentrates that can be diluted into the end user’s assay buffer of choice. Also supplied is a solution of the kit standard as a concentrate. The antibodies are not suitable for directly coating onto plates – they MUST be used with secondary antibody coated plates. Although these kits have been tested for cross reactivity and some species reactivity, individual labs will need to perform their own validations to account for species and laboratory differences. Please go to: https://www.arborassays.com/product_type/iswe-mini-kits/
Please keep the following information in mind when ordering mini-kits and Arbor Assays products.
- To receive the ISWE discount, the lab ordering the product must have at least one ISWE member. If the ISWE member leaves the lab, please have another one sign up as a member. A full ($150) or student ($75) two-year membership is a bargain for the discounts offered on Arbor Assays products, as well as the ISWE mini-kits.
- If your lab is located outside the USA, please order directly from Arbor Assays ([email protected]) rather than through your in-country distributor. The local distributor is not able to provide the ISWE discount or the ISWE price on mini-kits. Ordering directly does require prepayment as well as assuming responsibility for all processes and fees related to importing the products, including customs, duties, and VAT fees that are separate from the shipping charge. You should inform Arbor Assays during the ordering process if you have a FedEx, DHL, or UPS Collect Account that you would like to use for transport.
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Recommended Resources for Endocrine Methodologies
Bird Hormones and Bird Migrations: Analyzing Hormones in Droppings and Egg Yolks and Assessing Adaptations in Long-Distance Migration (2005). Annals of the New York Academy of Sciences, Volume 1046, Issue 1.
Special Issue: Non-Invasive Monitoring of Hormones (2013). Wiener Tierärztliche Monatsschrift – Veterinary Medicine Austria Volume 100. Click here to download the table of contents with links to full papers. This special issue recapped the 3rd ISWE Conference held in Vienna, Austria in 2012.
Hodges, K., Brown, J., Heistermann, M. (2010). Endocrine monitoring of reproduction and stress. In D.G. Kleiman, K.V. Thompson & C.K. Baer, Eds. Wild Mammals in Captivity: Principles and Techniques for Zoo Management. Chicago, IL: The University of Chicago Press, pp 447-468.
Jewgenow, K., Azevedo, A., Albrecht, M., Kirschbaum, C., & Dehnhard, M. (2020). Hair cortisol analyses in different mammal species: choosing the wrong assay may lead to erroneous results. Conservation Physiology, 8(1), coaa009.
Kalliokoski, O., Jellestad, F.K., Murison, R. (2019). A systematic review of studies utilizing hair glucocorticoids as a measure of stress suggests the marker is more appropriate for quantifying short-term stressors. Scientific Reports 9,11997. https://doi.org/10.1038/s41598-019-48517-2
MacDougall-Shackleton, S. A., Bonier, F., Romero, L. M., & Moore, I. T. (2019). Glucocorticoids and “stress” are not synonymous. Integrative Organismal Biology, 1(1), obz017.
Palme, R. (2019). Non-invasive measurement of glucocorticoids: Advances and problems. Physiology & Behavior, 199, 229-243.
Palme, R., Touma, C., Arias, N., Dominchin, M.F., Lepschy, M. (2013). Steroid extraction: Get the best out of faecal samples. Wiener Tierärztliche Monatsschrift – Veterinary Medicine Austria 100, 238-246.
Santymire, R. M., Manjerovic, M. B., & Sacerdote-Velat, A. (2018). A novel method for the measurement of glucocorticoids in dermal secretions of amphibians. Conservation Physiology, 6(1), coy008.
Sheriff, M. J., Dantzer, B., Delehanty, B., Palme, R., & Boonstra, R. (2011). Measuring stress in wildlife: Techniques for quantifying glucocorticoids. Oecologia, 166(4), 869-887.
Touma, C., & Palme, R. (2005). Measuring fecal glucocorticoid metabolites in mammals and birds: The importance of validation. Annals of the New York Academy of Sciences, 1046(1), 54-74.
Webber, J. T., Henley, M. D., Pretorius, Y., Somers, M. J., & Ganswindt, A. (2018). Changes in African elephant (Loxodonta africana) faecal steroid concentrations post-defaecation. Bothalia-African Biodiversity & Conservation, 48(2), 1-8.
Sample Analysis Resources
To add or edit contact information for this table, please contact [email protected].
|Lab Name||Website||Location||Country||Contact Name & Email||Contact Phone|
|Instituto de Investigaciones Biológicas y Tecnológicas (CONICET-UNC), Cordoba, Argentina||Website||Córdoba||Argentina||Ververs, DVM, Dipl ECAR||54-9-351-6711746|
|University of Veterinary Medicine, Vienna||Website||Vienna||Austria||Rupert Palme||43-1-25077-4103|
|Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Belgium||Website||Merelbeke||Belgium||Jella Wauters||0032478636862 (mobile) or 003292647452 (office)|
|University of Guelph||Website||Guelph, Ontario||Canada||Laura H Graham||519-546-6146|
|Reproductive Programs, Toronto Zoo||Website||Scarborough, Ontario||Canada||Gaby Mastromonaco||416-392-5980|
|Chester Zoo||Website||Upton-by-Chester||UK||Katie Edwards Or Rebecca Mogey||44-1244-389810 (office) or 44-1244-389447 (lab)|
|University of Edinburgh||Website||Edinburgh||UK||Forbes Howie||44-131-242-6612|
|Kari Morfeld||Omaha, NE||USA||Kari Morfeld||402-870-4458|
|Laboratory for the Evolutionary Endocrinology of Primates (LEEP) at the University of Arizona, in the School of Anthropology||Website||Tuscon, AZ||USA||Stacey Tecot||520-621-6294 (office) or 520-621-6367 (lab)|
|Saint Louis Zoo||Website||St Louis, MO||USA||Corinne Kozlowski||314-646-4762|
|Texas A&M University||Website||College Station, TX||USA||Annie Newell-Fugate||979-845-1744|
|Southeast Zoo Alliance for Reproduction and Conservation||Website||Yulee, FL||USA||Lara Metrione|
|University of Washington||Website||Seattle, WA||USA||Samuel Wasser Or Rebecca Booth||206-543-1669 or 206-543-1623|
|Smithsonian Conservation Biology Institute||Website||Front Royal, VA||USA||Janine Brown Or Steve Paris||540-635-6586 or 540-635-0052|
|Oregon Zoo||Website||Portland, OR||USA||Nadja Wielebnowski Or Candace Scarlata||503-226-1561 x 5301|
|Arbor Assays, including new assay development||Website||Ann Arbor, MI||USA||Bobbi O'Hara||1 734-677-1774|
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